INTERNO 1_2008_inglese

In vitro antibacterial efficacy of Vicryl plus suture (coated Polyglactin 910 with triclosan) using zone of inibition assays Objectives: This study evaluates the in vitro antimicrobial efficacy, of an absorbable suture coated with triclosan (Vicryl Plus) against two bacteria, potential responsible for the development of oral diseases: Pseudomonas aeruginosa and Streptococcus mutans. Methods: Vicryl Plus 3-0 and Vicryl 3-0 were tested for their efficiency against P. aeruginosa and S. Mutans. 27 segments 10 cm long each, of every suture, have been tested against P. Aeruginosa and S. Mutans respectively. Every sample has been dipped in a broth culture containing pure dried stocks of P. aeruginosa and S. mutans and placed in a Petri dish right after. Four hours later the sutures have been aseptically removed and placed in a selective culture. The incubation time was 18 hours for P. aeruginosa and 43 hours for S. mutans at 37°C. The antimicrobial efficacy of both sutures was performed by measuring the length of the bacteria-free suture segment. Results: A statistically significant difference between Vicryl Plus 3-0 and Vicryl 3-0 has been observed, with an higher bacterial growth on Vicryl 3-0 for both bacteria (P. aeruginosa and S. mutans). Conclusions: Vicryl Plus presented an antibacterial effectiveness in vitro against both P. aeruginosa and S. mutans.